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Preparation of Mica Supported Lipid Bilayers for High Resolution Optical Microscopy Imaging
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Bio-ingénierie
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Journal JoVE Bio-ingénierie
Preparation of Mica Supported Lipid Bilayers for High Resolution Optical Microscopy Imaging

Preparation of Mica Supported Lipid Bilayers for High Resolution Optical Microscopy Imaging

DOI:
10.3791/52054-v

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07:48 min

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June 07, 2014

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,
1School of Biological Sciences,Nanyang Technological University

Chapitres

  • 00:01Titre
  • 00:47Mica and Slides Preparation
  • 02:26Mica Assembly and Chamber Mounting
  • 05:10Supported Lipid Bilayer (SLB) Formation
  • 05:45Results: Diffusion Analysis of Glass and Mica Supported DOPC Bilayers
  • 07:11Conclusion

Summary

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We present a method of preparing mica supported lipid bilayers for high resolution microscopy. Mica is transparent and flat on an atomic scale, but rarely used in imaging because of handling difficulties; our preparation results in even deposition of the mica sheet, and reduces the material used in bilayer preparation.

Tags

Keywords: Supported Lipid BilayersMicaHigh-resolution Optical MicroscopySLB PreparationSLB ImagingLipid Vesicle DepositionCustom-made ChamberAFMSingle-molecule Fluorescence MicroscopyFCS

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