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Large-Scale Purification of Porcine or Bovine Photoreceptor Outer Segments for Phagocytosis Assays on Retinal Pigment Epithelial Cells
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Chapitres
- 00:05Titre
- 02:01Set-up, Solution and Gradient Preparation
- 03:20Tissue Collection and Retina Homogenization
- 04:54Photoreceptor Outer Segment Fragment Isolation
- 05:58POS Aliquoting Without and With Labeling
- 07:48Results: Large-scale Purification of Photoreceptor Outer Segments
- 09:28Conclusion
This article describes the protocol for the purification of photoreceptor outer segment fragments (POS) via ultracentrifugation from porcine/bovine retinae using homogenization and sucrose gradient centrifugation. This protocol allows the preparation of large stocks of POS aliquots, labeled or unlabeled, that can then be stored at -80 °C.
