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Chapitres
- 00:05Titre
- 02:09Preparation of Baby Hamster Kidney (BHK) Cell Culture Medium for Stem Cell Factor Collection
- 03:25EML Cell Culture and Depletion of Lineage Positive Cells
- 04:52Separation of Lin–CD34+ and Lin–CD34– Cells
- 07:15RNA Preparation and Library Construction for High-throughput Sequencing
- 09:54Results: RNA-Seq Identifies Transcription Factors that Regulate EML Self-renewal and Differentiation
- 11:38Conclusion
RNA-sequencing and bioinformatics analyses were used to identify significantly and differentially expressed transcription factors in Lin-CD34+ and Lin-CD34- subpopulations of mouse EMLcells. These transcription factors might play important roles in determining the switch between self-renewing Lin-CD34+ and partially differentiated Lin-CD34- cells.
