Journal
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Immunologie et infection
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A Novel In vitro Model for Studying the Interactions Between Human Whole Blood and Endothelium
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
A Novel In vitro Model for Studying the Interactions Between Human Whole Blood and Endothelium

A Novel In vitro Model for Studying the Interactions Between Human Whole Blood and Endothelium

DOI:
10.3791/52112-v

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06:27 min

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November 21, 2014

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, ,
1Department of Immunology, Genetics and Pathology,Uppsala University

Chapitres

  • 00:05Titre
  • 01:12Blood Endothelial Chamber Model
  • 04:14Results: The Effects of Blood Volume and TNFalpha on Blood Cell Recruitment and Activation of Coagulation
  • 05:59Conclusion

Summary

Traduction automatique

Traduction automatique

The accessibility of reliable models to investigate vascular blood interactions in humans is lacking. We present an in vitro model of cultured primary human endothelial cells combined with human whole blood to investigate cellular interactions both in the blood (ELISA) and the vascular compartment (microscopy).

Tags

Keywords: In Vitro ModelHuman Whole BloodEndotheliumCardiovascular PathologiesCoagulationThrombin Antithrombin ComplexesLeukocyte AttachmentTumor Necrosis FactorCD16+ Cells

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