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في تحليل المختبر من الاستجابة المناعية الخلوية بوساطة Myd88 لفيروس غرب النيل المسخ سلالة العدوى
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection
DOI:

10:39 min

November 27, 2014

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Chapitres

  • 00:05Titre
  • 01:40Flow Cytometry Analysis In Vitro OTII CD4+ T Cell Priming by DC from WNV-infected Mice
  • 05:00Intracellular Cytokine Staining
  • 07:13Results: DC Function and T Cell Responses at an Early Stage of WNV NS4B–P38G Infection
  • 09:45Conclusion

Summary

Traduction automatique

Two flow cytometry-based methods – an in vitro T cell priming assay and intracellular cytokine staining were utilized to measure antigen presenting capacity of dendritic cells and antigen-specific T cell responses to a West Nile virus mutant infection in mice.

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