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Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay
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Bio-ingénierie
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Journal JoVE Bio-ingénierie
Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay

Assessing Leukocyte-endothelial Interactions Under Flow Conditions in an Ex Vivo Autoperfused Microflow Chamber Assay

DOI:
10.3791/52130-v

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09:01 min

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December 30, 2014

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, ,
1Angiogenesis Laboratory, Department of Ophthalmology,Harvard Medical School, Massachusetts Eye & Ear Infirmary, 2St. Elizabeth’s Medical Center

Chapitres

  • 00:05Titre
  • 01:19Coating the Chamber
  • 02:35Filling the Chamber
  • 03:41Connecting the Chamber to the Mouse
  • 06:04Recording Rolling Velocity
  • 07:32Results: Representative Time Course of Leukocytes Rolling Through the Flow Chamber
  • 08:35Conclusion

Summary

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Traduction automatique

Here, we present a protocol that allows the investigator to assess leukocyte recruitment dynamics ex vivo by connecting a chamber coated with endothelial-derived adhesion molecules to the circulatory system of a mouse. This method offers significant advantages since it allows for leukocyte assessment under relative biological conditions.

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Keywords: Leukocyte-endothelial InteractionsEx Vivo Autoperfused Microflow Chamber AssayInflammationPeripheral Blood Leukocytes (PBLs)Adhesion MoleculesIntravital MicroscopyFlow ConditionsLeukocyte Rolling VelocityImageJ

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