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Fluorescens-quenching af et liposomalt indkapslet Near-infrarøde fluorofor som et værktøj til<em> In Vivo</em> Optisk Imaging
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Bio-ingénierie
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Journal JoVE Bio-ingénierie
Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging

Fluorescens-quenching af et liposomalt indkapslet Near-infrarøde fluorofor som et værktøj til<em> In Vivo</em> Optisk Imaging

DOI:
10.3791/52136-v

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10:55 min

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January 05, 2015

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, , , , ,
1Experimental Radiology, Institute of Diagnostic and Interventional Radiology I,Jena University Hospital, 2Department of Pharmaceutical Technology,Friedrich-Schiller-University Jena, 3Center for Electron Microscopy,Jena University Hospital

Chapitres

  • 00:05Titre
  • 02:08Preparation of Spontaneously Formed Vesicle (SFV) Dispersion
  • 03:54Extrusion and Purification of Homogeneous Liposome Vesicles Encapsulating DY-676-COOH
  • 05:21Physico-chemical Analysis of Flourescence Quenching and Activation
  • 07:10Induction of Inflammation and In Vivo NIRF Imaging
  • 08:10Results: Representative Liposomal Flourescence Quenching Analyses
  • 09:54Conclusion

Summary

Traduction automatique

Traduction automatique

The use of fluorophores for in vivo imaging can be greatly limited by opsonization, rapid clearance, low detection sensitivity and cytotoxic effects on the host. Encapsulation of fluorophores in liposomes by film hydration and extrusion leads to fluorescence quenching and protection which enables in vivo imaging with high detection sensitivity.

Tags

Keywords: Fluorescence QuenchingLiposomal EncapsulationNear-infrared FluorophoreIn Vivo Optical ImagingInflammation ImagingZymosan-induced Edema ModelEPR EffectLiposome Formulations

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