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Regioselective Biolistic Targeting in Organotypic Brain Slices Using a Modified Gene Gun
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Regioselective Biolistic Targeting in Organotypic Brain Slices Using a Modified Gene Gun

Regioselective Biolistic Targeting in Organotypic Brain Slices Using a Modified Gene Gun

DOI:
10.3791/52148-v

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06:40 min

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October 24, 2014

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, , ,
1Leslie Dan Faculty of Pharmacy,University of Toronto, 2MRC-Laboratory of Molecular Biology, Cambridge, UK

Chapitres

  • 00:05Titre
  • 01:21Preparation of Organotypic Slices
  • 02:20Preparation of DNA-coated Micro & Nano-projectiles
  • 03:38Biolistic Transfection on Organotypic Slices
  • 04:29Fixing and Visualization of Brain Slices
  • 05:24Results: Biolistic Delivery of Fluorescent Protein Encoded DNA into Organotypic Slices
  • 06:12Conclusion

Summary

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Traduction automatique

Recent improvements in organotypic brain slice preparations have permitted their exploitation for biotechnological applications. Organotypic slices maintain local structural characteristics of in vivo biology, including functional synaptic connections. Here we present a regioselective biolistic delivery method to label and genetically manipulate these slices.

Tags

Keywords: Biolistic TargetingOrganotypic Brain SlicesModified Gene GunDNA TransfectionRegioselective TransfectionTerminally Differentiated NeuronsTransient ExpressionStable ExpressionNanoparticle BombardmentEpisomal ExpressionAnatomical IsolationHeterologous Gene Effects

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