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Médecine
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Ex Vivo Treatment Response of Primary Tumors and/or Associated Metastases for Preclinical and Clinical Development of Therapeutics
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Médecine
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Journal JoVE Médecine
Ex Vivo Treatment Response of Primary Tumors and/or Associated Metastases for Preclinical and Clinical Development of Therapeutics

Ex Vivo Treatment Response of Primary Tumors and/or Associated Metastases for Preclinical and Clinical Development of Therapeutics

DOI:
10.3791/52157-v

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08:29 min

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October 02, 2014

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, , , , , , ,
1Department of Pathology,Memorial Sloan Kettering Cancer Center, 2Molecular Pharmacology and Chemistry Program,Memorial Sloan Kettering Cancer Center, 3Department of Radiology,Weill Cornell Medical College, 4Department of Medicine,Memorial Sloan Kettering Cancer Center, 5Department of Oncology,Memorial Sloan Kettering Cancer Center, 6Department of Surgery,Memorial Sloan Kettering Cancer Center

Chapitres

  • 00:05Titre
  • 02:30Tissue Procurement
  • 04:09Preparation of Materials for Sectioning and Vibratome Settings
  • 05:19Tissue Sectioning, Treatment, and Analyses
  • 06:25Results: Ex Vivo Metastatic Breast Cancer Response to Heat Shock Protein 90 Inhibitor
  • 07:56Conclusion

Summary

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Traduction automatique

Established cancer cell lines and xenografts have been the mainstay of cancer research for the past several decades. However, recent evidence suggests that therapeutic response is greatly influenced by the tumor cell microenvironment. Therefore, we have developed an ex vivo analysis of primary tumor specimens for drug development purposes.

Tags

Ex Vivo Tumor ModelPrimary Tumor SamplesTumor MicroenvironmentTreatment ResponsePreclinical Drug DevelopmentClinical TherapeuticsCancer Cell LinesTumor XenograftsTumor-microenvironment Crosstalk

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