/
/
افتعال ركائز الثقافة معقدة باستخدام الروبوتية Microcontact الطباعة (R-μCP) وتبديل أليف النواة متسلسل
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 01:49Surface-initiated Atom Transfer Radical Polymerization (SI-ATRP) of PEGMEMA
- 03:15Functionalization of Micropatterned PEGMEMA Chains
- 05:11“Click” Biotinylation of PEGMEMA Chains Including Staining
- 07:10Results: System Accuracy
- 07:57Conclusion
Cell culture substrates functionalized with microscale patterns of biological ligands have immense utility in the field of tissue engineering. Here, we demonstrate the versatile and automated manufacture of tissue culture substrates with multiple, micropatterned poly(ethylene glycol) brushes presenting orthogonal chemistries that enable spatially precise and site-specific immobilization of biological ligands.
