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बृहतभक्षककोशिका Phagocytosis और actin polymerization के आकलन के लिए उच्च Throughput Fluorometric तकनीक
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization
DOI:

09:22 min

November 27, 2014

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Chapitres

  • 00:05Titre
  • 01:38Plating and Treating Cells
  • 02:16Opsonization of Flourescent Particles
  • 03:36Phagocytosis
  • 05:08Actin Staining
  • 06:10Quantification
  • 07:33Results: Confocal Microscopy of Phagocytosis and Actin Polymerization
  • 08:47Conclusion

Summary

Traduction automatique

Here we present a protocol to quantify phagocytosis of fluorescent particles by adherent macrophage cell line using a fluorometric method. This method facilitates a high throughput quantification of particle internalization as well as the resulting actin polymerization.

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