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En Prosedyre for Implantere en Spinal Chamber for Longitudinal<em> I Vivo</em> Imaging av Mouse Spinal Cord
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
A Procedure for Implanting a Spinal Chamber for Longitudinal In Vivo Imaging of the Mouse Spinal Cord

En Prosedyre for Implantere en Spinal Chamber for Longitudinal<em> I Vivo</em> Imaging av Mouse Spinal Cord

DOI:
10.3791/52196-v

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11:20 min

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December 03, 2014

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1Department of Neurobiology and Behavior,Cornell University, 2Department of Biomedical Engineering,Cornell University

Chapitres

  • 00:05Titre
  • 01:56Preparing the Mouse for Surgery
  • 02:56Expose the Dorsal Laminae of Thoracic Vertebrae
  • 05:11Perform a Dorsal Laminectomy
  • 06:54Seal the Chamber
  • 09:16Results: Chronic Spinal Chamber Allows Repeated Multi-photon Imaging
  • 10:56Conclusion

Summary

Traduction automatique

Traduction automatique

I denne videoen beskriver vi en prosedyre for å implantere en kronisk optisk avbildning salen over dorsal ryggmargen av en levende mus. Kammeret, kirurgisk prosedyre, og kronisk bildebehandling blir gjennomgått og demonstrert.

Tags

Keywords: Spinal ChamberIn Vivo ImagingMouse Spinal CordSurgical ProcedureOptical AccessNonlinear MicroscopyNeurodegenerative InsultsChronic ImagingVertebral ClampingSurgical ImplantPost-operative CareImaging Stability

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