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개발<em> 체외</em의 상호 작용을 연구> 모델 시스템<em> 에쿠스</em><em> caballus</em높은 친 화성 수용체 FcεRI에>의 IgE
Journal JoVE
Immunologie et infection
Author Produced
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Journal JoVE Immunologie et infection
Development of an in vitro model system for studying the interaction of Equus caballus IgE with its high-affinity receptor FcεRI
DOI:

07:31 min

November 01, 2014

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Chapitres

  • 00:00Titre
  • 00:10Introduction
  • 01:14Animation
  • 02:37Washing
  • 03:22Antigen Challenge
  • 03:48Substrate
  • 04:23Stopping the Reaction
  • 04:47Reading
  • 04:54Calculations
  • 06:22Résultats
  • 07:02Conclusion

Summary

Traduction automatique

The current study describes the development and applications of a genetically engineered assay system based on the transfection of rat basophilic leukemia cells with the equine FcεRIα gene. Transfected cells express a functional receptor where the release of mediators of the allergic response can be activated by IgE and antigen.

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