This content is Free Access.
Chapitres
- 00:05Titre
- 01:31Preparation of the Gastrointestinal Tract and Dissection of the Midgut
- 03:14Digestion of the Midgut Tissue to Harvest Cells for FAC Sorting
- 05:25FACS to Isolate Intestinal Stem Cells (ISC)
- 08:52Results: Representative Analyses of the Sorted Cells
- 10:17Conclusion
Understanding the endogenous molecular changes in adult stem cells during aging requires isolating the cells of interest. The method described here presents a simple and robust approach to enrich for and isolate Drosophila intestinal stem cells and the enteroblast progenitor cells by FACS at any time point during aging.
