对专为递送治疗因素的神经保护策略成人干细胞高通量表征

Journal JoVE
Médecine

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Journal JoVE Médecine

High Throughput Characterization of Adult Stem Cells Engineered for Delivery of Therapeutic Factors for Neuroprotective Strategies

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对专为递送治疗因素的神经保护策略成人干细胞高通量表征

DOI:

10.3791/52242-v

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09:19 min

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January 04, 2015

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Anup D. Sharma, Pavel A. Brodskiy³, Emma M. Petersen³, Melih Dagdeviren, Eun-Ah Ye, Surya K. Mallapragada, Donald Sakaguchi³

¹Department of Chemical and Biological Engineering,Iowa State University, ²Department of Genetics, Development and Cell Biology,Iowa State University, ³Biology Program,Iowa State University

Chapitres

00:05Titre
01:45Substrate Preparation and Cell Plating
03:35Time Lapse Imaging
05:01Ki67 Cell Proliferation, Automated Imaging and Multi-wavelength Scoring Analysis
07:00Results: Characterization of Engineered MSCs
08:29Conclusion

Summary

Traduction automatique

This study describes an experimental platform to rapidly characterize engineered stem cells and their behaviors before their application in long-term in vivo transplant studies for nervous system rescue and repair.

对专为递送治疗因素的神经保护策略成人干细胞高通量表征

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Chapitres

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