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Utföra subretinal injektioner i Gnagare att leverera pigmentepitelet celler i suspension
Journal JoVE
Médecine
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Journal JoVE Médecine
Performing Subretinal Injections in Rodents to Deliver Retinal Pigment Epithelium Cells in Suspension
DOI:

06:04 min

January 23, 2015

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Chapitres

  • 00:05Titre
  • 01:15RPE Cell Preparation for Injection
  • 02:36Sub-retinal Injection
  • 04:29Results: RPE Subretinal Delivery
  • 05:23Conclusion

Summary

Traduction automatique

Here we present a community accepted protocol in multimedia format for subretinally injecting a bolus of RPE cells in rats and mice. This approach can be used for determining rescue potentials, safety profiles, and survival capacities of grafted RPE cells upon implantation in animal models of retinal degeneration.

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