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La valutazione delle proprietà sulle cellule staminali in cellule di carcinoma ovarico umano Utilizzo multi e single cell-based Spheres Assays
Journal JoVE
Médecine
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Journal JoVE Médecine
Evaluation of Stem Cell Properties in Human Ovarian Carcinoma Cells Using Multi and Single Cell-based Spheres Assays
DOI:

08:39 min

January 03, 2015

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Chapitres

  • 00:05Titre
  • 01:35Cell Sorting and Plating
  • 04:16Serial Sphere Passaging
  • 05:59Results: Representative RFP+ and RFP- tumor Sphere Imaging
  • 07:56Conclusion

Summary

Traduction automatique

In vitro spheres assays are commonly used to identify cancer stem cells. Here we compare single with multi cell-based spheres assays. The more laborious single cell-based assays or methylcellulose supplementation give more accurate results while multi cell-based assays performed in liquid medium can be highly influenced by cell density.

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