Journal
/
Biologie du développement
/
Deriving Retinal Pigment Epithelium (RPE) from Induced Pluripotent Stem (iPS) Cells by Different Sizes of Embryoid Bodies
Journal JoVE
Biologie du développement
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie du développement
Deriving Retinal Pigment Epithelium (RPE) from Induced Pluripotent Stem (iPS) Cells by Different Sizes of Embryoid Bodies

Deriving Retinal Pigment Epithelium (RPE) from Induced Pluripotent Stem (iPS) Cells by Different Sizes of Embryoid Bodies

DOI:
10.3791/52262-v

•

09:18 min

•

February 04, 2015

•

, , , ,
1Ocular Trauma,U.S. Army Institute of Surgical Research

Chapitres

  • 00:05Titre
  • 01:52Induced Pluripotent Stem (iPS) Cell Culture and Passaging
  • 03:39Generation, Plating, and Differentiation of Embryoid Bodies (EBs)
  • 06:28Results: Representative Retinal Pigment Epithelium (RPE)
  • 08:11Conclusion

Summary

Traduction automatique

Traduction automatique

The objective of this report is to describe the protocols to derive the retinal pigment epithelium (RPE) from induced pluripotent stem (iPS) cells using different sizes of embryoid bodies.

Tags

Induced Pluripotent Stem CellsRetinal Pigment EpitheliumEmbryoid BodiesDifferentiationMicrowell PlatesImmunocytochemistryRT-PCRFACS Analysis

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Efficient Derivation of Retinal Pigment Epithelium Cells from Stem Cells

Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies

Differentiating Chondrocytes from Peripheral Blood-derived Human Induced Pluripotent Stem Cells

Chondrogenic Pellet Formation from Cord Blood-derived Induced Pluripotent Stem Cells

Rapid, Directed Differentiation of Retinal Pigment Epithelial Cells from Human Embryonic or Induced Pluripotent Stem Cells

Engineering Transplantation-suitable Retinal Pigment Epithelium Tissue Derived from Human Embryonic Stem Cells

Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells

Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem Cells

In Vitro Generation of Somite Derivatives from Human Induced Pluripotent Stem Cells

Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition

Read Article