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Dissecção e montagem de<em> Drosophila</em> pupais Discos Eye
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Biologie
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Journal JoVE Biologie
Dissection and Mounting of Drosophila Pupal Eye Discs

Dissecção e montagem de<em> Drosophila</em> pupais Discos Eye

DOI:
10.3791/52315-v

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07:48 min

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November 09, 2014

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, , , ,
1Department of Molecular, Cell and Developmental Biology (MCDB),University of California at Los Angeles (UCLA), 2Arizona College of Osteopathic Medicine (AZCOM),Midwestern University, 3MCDB, Broad Stem Cell Research Center,UCLA

Chapitres

  • 00:00Titre
  • 00:12Dissection Overview
  • 00:36Dissection of the Eye Disc
  • 03:45Fixing and Immunostaining
  • 04:27Mounting
  • 06:45Antibody Stained Sye Discs
  • 07:17Conclusion

Summary

Traduction automatique

Traduction automatique

The goal of this technique is to enable researchers to perform dissection, immunostaining and mounting of pupal eye discs from Drosophila melanogaster of any age.

Tags

Keywords: DrosophilaEye DiscPupal PhaseOmmatidiumPhotoreceptorCone CellPigment CellBristle CellMorphogenetic FurrowDifferentiationImmunostaining

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