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SDF-1α - 壳聚糖硫酸葡聚糖纳米粒子的制备与表征
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Chimie
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Journal JoVE Chimie
Preparation and Characterization of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles
DOI:

12:00 min

January 22, 2015

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Chapitres

  • 00:05Titre
  • 01:22Preparation of Stromal Cell-derived Factor-1α (SDF-1α) Glycan Nanoparticles
  • 03:34Measurement of Particle Size and Zeta Potential
  • 05:07Quantification of SDF-1α in the Particles
  • 06:26In Vitro Release Assay
  • 07:38Migration Assay
  • 09:19Results: Analysis of SDF-1α-Chitosan-Dextran Sulfate Nanoparticles
  • 11:23Conclusion

Summary

Traduction automatique

The objective of this protocol is to incorporate SDF-1α, a stem cell homing factor, into dextran sulfate-chitosan nanoparticles. The resultant particles are measured for their size and zeta potential, as well as the content, activity, and in vitro release rate of SDF-1α from the nanoparticles.

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