Journal
/
Biologie du développement
/
基于发育毒性测定化学品安全筛选和系统生物学数据生成人类多能干细胞
Journal JoVE
Biologie du développement
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie du développement
Human Pluripotent Stem Cell Based Developmental Toxicity Assays for Chemical Safety Screening and Systems Biology Data Generation
Please note that all translations are automatically generated. Click here for the English version.

基于发育毒性测定化学品安全筛选和系统生物学数据生成人类多能干细胞

DOI:
10.3791/52333-v

•

17:28 min

•

June 17, 2015

•

, , , , , , , , , , ,
1Center of Physiology and Pathophysiology, Institute of Neurophysiology,University of Cologne, 2Department of Biology,University of Konstanz, 3Department of Statistics,Technical University of Dortmund, 4Leibniz Research Centre for Working Environment and Human Factors,Technical University of Dortmund

Chapitres

  • 00:05Titre
  • 02:02H9 Cell Culture on Basement Membrane Matrix Coated Plates
  • 04:40Embryoid Bodies Formation
  • 06:17Collection of Embryoid Bodies and Treatment
  • 07:55RNA Isolation
  • 09:31Splitting and Maintenance of H9 Cells
  • 10:55Differentiation of hESC towards Neuroectodermal Progenitor Cells (NEP)
  • 15:06Results: Methyl Mercury Exposure Causes Gene Expression Changes in Embryoid Bodies
  • 16:40Conclusion

Summary

Traduction automatique

Traduction automatique

该协议描述了基于人胚胎干细胞和转录研究2 在体外发育毒性试验系统（UKK和UKN1）。测试系统预测人类发育毒性危险，并可能有助于减少动物研究中，成本和所需的化学安全测试的时间。

Tags

Human Pluripotent Stem CellsDevelopmental Toxicity AssaysChemical Safety ScreeningSystems BiologyEmbryonic Stem CellsEmbryoid BodiesNeuroectodermal Progenitor CellsTranscriptome MicroarrayIn Vitro Toxicity TestingEarly Embryonic ToxicityTeratogenicityDevelopmental NeurotoxicityEpigenetic ChangesToxicity BiomarkersSystems Biology AnalysisAnimal Studies ReductionDrug DevelopmentChemical Safety Evaluation

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/52333?article_type=v&slug=human-pluripotent-stem-cell-based-developmental-toxicity-assays-for

转基因器官皮肤培养使用灭活人真皮代
check_url/fr/52333?article_type=v&slug=human-pluripotent-stem-cell-based-developmental-toxicity-assays-for

培养人多能性和神经干细胞的基本和临床前研究一个封闭的细胞培养系统
check_url/fr/52333?article_type=v&slug=human-pluripotent-stem-cell-based-developmental-toxicity-assays-for

从人多能干细胞的黑素细胞的无饲养推导
check_url/fr/52333?article_type=v&slug=human-pluripotent-stem-cell-based-developmental-toxicity-assays-for

从人多能干细胞使用高度可重复的小分子为基础的分化协议心肌细胞规模化生产
check_url/fr/52333?article_type=v&slug=human-pluripotent-stem-cell-based-developmental-toxicity-assays-for

从人体外周血单个核细胞融合，无诱导多能干细胞的生成方法游离型载体
check_url/fr/52333?article_type=v&slug=human-pluripotent-stem-cell-based-developmental-toxicity-assays-for

界定和由人多能干细胞的肝细胞样细胞的可扩展代
check_url/fr/52333?article_type=v&slug=human-pluripotent-stem-cell-based-developmental-toxicity-assays-for

人胚胎或诱导多能干细胞对视网膜色素上皮细胞的快速定向分化
check_url/fr/52333?article_type=v&slug=human-pluripotent-stem-cell-based-developmental-toxicity-assays-for

改良多向分化效力的常规人多能干细胞的化学逆转与幼稚样态
check_url/fr/52333?article_type=v&slug=human-pluripotent-stem-cell-based-developmental-toxicity-assays-for

基于 rna 的人原代成纤维细胞重组成诱导多能干细胞
check_url/fr/52333?article_type=v&slug=human-pluripotent-stem-cell-based-developmental-toxicity-assays-for

人类多能干细胞有效分化为肝细胞

Read Article