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Chapitres
- 00:05Titre
- 01:49Passaging and Maintenance of Embryonic Stem Cells (ESCs)
- 03:42Neuronal Differentiation
- 08:26Measured Inhibition of Synaptic Transmission (MIST) Assay for Quantifying Miniature Excitatory Post-synaptic Currents
- 12:02Results: Synaptic Activity in ESNs Measured by the MIST Assay
- 14:13Conclusion
A custom protocol is described to differentiate mouse ES cells into defined populations of highly pure neurons exhibiting functioning synapses and emergent network behavior. Electrophysiological analysis demonstrates the loss of synaptic transmission following exposure to botulinum neurotoxin serotypes /A-/G and tetanus neurotoxin.
