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Radial Mobility and Cytotoxic Function of Retroviral Replicating Vector Transduced, Non-adherent Alloresponsive T Lymphocytes
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1Department of Neurosurgery,UCLA David Geffen School of Medicine, 2Department of Molecular and Medical Pharmacology,UCLA David Geffen School of Medicine, 3Department of Medicine,UCLA David Geffen School of Medicine, 4Brain Research Institute,UCLA David Geffen School of Medicine, 5Jonsson Comprehensive Cancer Center,UCLA David Geffen School of Medicine
Chapitres
- 00:05Titre
- 01:58Seeding the Slides with Adherent Tumor Cells
- 04:05Sedimentation of the Non-adherent T Cells onto the Tumor Cell Monolayer
- 05:49Data Acquisition, Analysis, and Display
- 07:41Results: Migration of Alloreactive Cytotoxic T Lymphocytes across Tumor Cell Monolayers
- 08:55Conclusion
We describe a protocol to monitor radial mobility of non-adherent immune cells in vitro using a cell sedimentation manifold/slide apparatus. Cell migration is tracked on monolayers of tumor cells or on extracellular matrix proteins. Examination by light and fluorescence microscopy allows for observation of cell mobility and cytotoxic functionality.
