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Optagelse Light-fremkaldte Postsynaptiske Responses i neuroner i Dark-tilpassede, Mouse Retinal Slice Forberedelser Brug Patch Clamp Techniques
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Neurosciences
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Journal JoVE Neurosciences
Recording Light-evoked Postsynaptic Responses in Neurons in Dark-adapted, Mouse Retinal Slice Preparations Using Patch Clamp Techniques

Optagelse Light-fremkaldte Postsynaptiske Responses i neuroner i Dark-tilpassede, Mouse Retinal Slice Forberedelser Brug Patch Clamp Techniques

DOI:
10.3791/52422-v

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10:30 min

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February 11, 2015

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1Anatomy and Cell Biology,Wayne State University School of Medicine, 2Ophthalmology,Wayne State University School of Medicine

Chapitres

  • 00:05Titre
  • 01:19Retinal Dissection and Slice Preparation
  • 06:10Patch Clamp Recordings
  • 09:23Results: Light-evoked Postsynaptic Responses
  • 10:07Conclusion

Summary

Traduction automatique

Traduction automatique

We will demonstrate how to prepare retinal slices from the mouse eye and record light responses in retinal neurons. The entire procedure is conducted in dark-adapted conditions.

Tags

Keywords: RetinaVisual SystemNeural NetworkRetinal NeuronsBipolar CellsGanglion CellsAmacrine CellsPatch ClampDark-adaptedMouse Retinal SliceLight-evoked Synaptic ResponsesVisual Signal Processing

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