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Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS
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Médecine
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Journal JoVE Médecine
Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS

Quantification of the Immunosuppressant Tacrolimus on Dried Blood Spots Using LC-MS/MS

DOI:
10.3791/52424-v

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08:38 min

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November 08, 2015

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, , , , , , ,
1iC42 Clinical Research and Development,University of Colorado, Anschutz Medical Campus, 2Division of Clinical Pharmacology,Cincinnati Children’s Hospital Medical Center, 3Food and Drug Administration (FDA),Center of Drug Evaluation Research – Office of Generic Drugs, 4Transplant Clinical Research,University of Cincinnati

Chapitres

  • 00:05Titre
  • 01:05Preparation of References, Solutions, Calibration Curve and Quality Control Samples
  • 03:52Collection of Clinical Samples and Extraction of Tacrolimus Dried Blood Spot Samples
  • 05:02LC-MS/MS Analysis and Quantification
  • 06:20Results: Analysis and Quantification of Tacrolimus on Dried Blood Spots by LC-MS/MS
  • 08:01Conclusion

Summary

Traduction automatique

Traduction automatique

Here we describe a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) assay to quantify the immunosuppressant tacrolimus in dried blood spots using a simple manual protein precipitation step and online column extraction.

Tags

Keywords: TacrolimusImmunosuppressantLC-MS/MSDried Blood SpotsTherapeutic Drug MonitoringSolid Organ TransplantationProtein PrecipitationOnline Column ExtractionHPLCMass SpectrometryAssay ValidationStability

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