Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 01:44Protein Degradation Mutant Identification Using a Yeast Growth Assay
- 05:06Biochemical Confirmation of Protein Degradation Defects
- 08:29Results: Ubiquitin Ligase-dependent Degradation of an Unstable Protein Fused to a Metabolic Reporter
- 10:18Conclusion
This article describes a yeast growth-based assay for the determination of genetic requirements for protein degradation. It also demonstrates a method for rapid extraction of yeast proteins, suitable for western blotting to biochemically confirm degradation requirements. These techniques can be adapted to monitor degradation of a variety of proteins.
