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Macrofagi Colesterolo Depletion e il suo effetto sulla fagocitosi di<em> Cryptococcus neoformans</em
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Macrophage Cholesterol Depletion and Its Effect on the Phagocytosis of Cryptococcus neoformans
DOI:

11:07 min

December 19, 2014

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Chapitres

  • 00:05Titre
  • 01:54Cholesterol Depletion of J774A.1 Cells with MβCD
  • 03:26Infection of Macrophages with C. neoformans H99
  • 08:20Results: C. neoformans Infection of Cholesterol-depleted Cells
  • 10:02Conclusion

Summary

Traduction automatique

In this article, a protocol for infection of macrophages with Cryptococcus neoformans is described. Also, a method for sterol depletion from the macrophages is explained. These protocols provide a guide to study fungal infections in vitro and examine the role of sterols in such infections.

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