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EPA Método 1615. A medição do Enterovirus e Norovirus Ocorrência na água por cultura e RT-qPCR. II. De ensaio total de Vírus cultiváveis
Journal JoVE
Environnement
This content is Free Access.
Journal JoVE Environnement
EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. II. Total Culturable Virus Assay
DOI:

11:09 min

September 11, 2016

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Chapitres

  • 00:05Titre
  • 02:32Filter Elution Procedure
  • 03:33Organic Flocculation Concentration Procedure
  • 06:03Total Culturable Virus Quantal Assay
  • 09:26Results: Mean Poliovirus Recovery (%) from Ground and Reagent-grade Water
  • 10:19Conclusion

Summary

Traduction automatique

Here we present a procedure to measure total culturable viruses using the Buffalo Green Monkey kidney cell line. The procedure provides a standardized tool for measuring the occurrence of infectious viruses in environmental and drinking waters.

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