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Chapitres
- 00:05Titre
- 01:28H+ Killing Procedure
- 02:21Measurement of Intracellular pH
- 04:16Initial Rate NHE7 Measurement by Fast Li+ Uptake
- 05:42Results: Representative Selection and Functional Characterization
- 07:09Conclusion
The first part of this article shows how to select mutant cell lines expressing vesicular Na+/H+ exchangers at their plasma membrane. The second part provides protocols based on intracellular pH measurements and fast ion uptake, which are used to determine the ion selectivity and the kinetic parameters of these exchangers.
