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Mechanische vatverwonding in zebravis embryo's

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Biologie du développement

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Journal JoVE Biologie du développement

Mechanical Vessel Injury in Zebrafish Embryos

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Mechanische vatverwonding in zebravis embryo's

DOI:

10.3791/52460-v

•

06:38 min

•

February 17, 2015

•

Hilary Clay, Shaun R. Coughlin

¹Cardiovascular Research Institute,University of California

Chapitres

00:05Titre
01:07Preparation of Tools and Zebrafish Embryos for Injury
02:22Mechanical Vessel Injury of Embryos
03:33Analysis of Hemostasis and Wound Healing
05:02Results: Coagulation in Response to Mechanical Vessel Injury
06:17Conclusion

Summary

Traduction automatique

This article describes a method for creating a mechanical vessel injury in zebrafish embryos. This injury model provides a platform for studying hemostasis, injury-related inflammation, and wound healing in an organism ideally suited for real-time microscopy.

Tags

Keywords: Zebrafish Embryos Mechanical Vessel Injury Vascular Injury Hemostasis Wound Repair In Vivo Microscopy Transgenic Forward And Reverse Genetics Pharmacological Manipulation

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