Journal
/
/
指南生成和使用hiPSC派生的NPC的神经系统疾病的研究
Journal JoVE
Médecine
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Médecine
A Guide to Generating and Using hiPSC Derived NPCs for the Study of Neurological Diseases
DOI:

09:30 min

February 21, 2015

, ,

Chapitres

  • 00:05Titre
  • 01:22Harvest of Neural Rosettes
  • 03:03Expansion of Neural Progenitor Cells
  • 04:27NPC Transduction
  • 05:15Neurosphere Migration Assay
  • 07:06Results: Patient-specific hiPSCs can be Differentiated into Healthy NPCs and Neurons
  • 08:56Conclusion

Summary

Traduction automatique

This protocol describes how neural progenitor cells can be differentiated from human induced pluripotent stem cells, in order to yield a robust and replicative neural cell population, which may be used to identify the developmental pathways contributing to disease pathogenesis in many neurological disorders.

Vidéos Connexes

Read Article