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ומניפולציה<em> במבחנה</em> ההבשלה של<em> Laevis Xenopus</em> ביציות, ואחריו הזרקת Intracytoplasmic זרע, לחקר התפתחות העוברית
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Chapitres
- 00:05Titre
- 01:14Preparations
- 03:47Injecting Oocytes with Oligos and Oocyte Maturation
- 05:55Intracytoplasmic Sperm Injection
- 08:12Results: ICS Embryo Survival
- 09:01Conclusion
We describe methods of manipulating Xenopus laevis immature oocytes, in vitro maturation of oocytes to eggs, and intracytoplasmic sperm injection. This protocol allows degradation of some maternal proteins and overexpression of genes of interest at fertilization, and hence is valuable to study roles of specific factors in early embryonic development.
