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Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq
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Chapitres
- 00:05Titre
- 01:37Chromatin Cross-linking
- 02:55Chromatin Extraction
- 04:31Chromatin Fragmentation
- 06:07Chromatin Immunoprecipitation
- 08:16Results: Chromatin Binding of the T-box Transcription Factor VegT at Gastrula Stage of X. tropicalis Embryos
- 09:54Conclusion
The question of how chromatin regulators and chromatin states affect the genome in vivo is key to our understanding of how early cell fate decisions are made in the developing embryo. ChIP-Seq—the most popular approach to investigate chromatin features at a global level—is outlined here for Xenopus embryos.
