Journal
/
/
在大鼠脑组织用荧光活化细胞分选,以检查细胞类型特异性基因表达
Journal JoVE
Neurosciences
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Neurosciences
Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue
DOI:

08:37 min

May 28, 2015

Chapitres

  • 00:05Titre
  • 01:09Neural Dissociation
  • 03:14Myelin Depletion
  • 04:50FACS Staining
  • 06:27Results: Sorting Neurons, Astrocytes, and Microglia from Individual Samples
  • 08:11Conclusion

Summary

Traduction automatique

The goal of this protocol is to use the fluorescence activated cell sorting (FACS) technique to sort specific types of neural cells for subsequent analysis of cell-type-specific gene expression, epigenetic markers, and or protein expression.

Vidéos Connexes

Read Article