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En metod för att välja Struktur-växling Aptamerer Tillämpat på en kolorimetrisk Gold nanopartiklar analys
Journal JoVE
Biologie
This content is Free Access.
Journal JoVE Biologie
A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
DOI:

12:31 min

February 28, 2015

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Chapitres

  • 00:05Titre
  • 01:41Bead Preparations
  • 04:03The First Round of Selection
  • 05:36Monitoring and Amplifying Product between Selection Rounds
  • 08:40Subsequent Rounds of Selection
  • 09:51The AuNP Assay
  • 10:33Generation of Aptamers to Cortisol
  • 11:57Conclusion

Summary

Traduction automatique

A protocol is provided to select structure-switching aptamers for small molecule targets based on a tunable stringency magnetic bead selection method. Aptamers selected with structure-switching properties are beneficial for assays that require a conformational change to signal the presence of a target, such as the described gold nanoparticle assay.

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