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Ex Utero Electroporation og organotypiske Slice Culture of Mouse hippocampus Tissue
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Chapitres
- 00:05Titre
- 01:17DNA Injection and Electroporation of E15.5 and E18.5 Embryos
- 03:13Dissection of the Brain
- 04:21Vibratiome Sectioning and Slice Culture
- 06:07Fixation of the Sections Followed by Immunofluorescence Staining
- 07:48Results: Ex Utero Electroporation at E18.5 Following by Organotypic Slice Culture
- 08:49Conclusion
Here we present a protocol providing a tool to examine regulatory mechanisms of specific genes during hippocampal development. Employing ex utero electroporation and organotypic slice culture allows the up- and down-regulation of the expression of genes of interest in single cells and follow their fate during development.
