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Lymfocyt Isolatie van Human Skin voor Fenotypische Analyse en<em> Ex Vivo</em> Cell Culture
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Lymphocyte Isolation from Human Skin for Phenotypic Analysis and Ex Vivo Cell Culture
DOI:

10:31 min

April 08, 2016

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Chapitres

  • 00:05Titre
  • 01:19Preparation of Single Cell Suspensions from Human Skin
  • 04:54Polyclonal Activation of Skin-resident T Cells and Flow Cytometry Analysis
  • 08:04Results: Phenotypic Analysis of Skin-derived Lymphocytes
  • 10:10Conclusion

Summary

Traduction automatique

We describe a protocol to efficiently isolate skin resident T cells from human skin biopsies. This protocol yields sufficient numbers of viable human skin resident lymphocytes for flow cytometric analysis and ex vivo culture.

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