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Gold-Nanostäbchenunterstützte optische Stimulation von Nervenzellen
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Gold Nanorod-assisted Optical Stimulation of Neuronal Cells
DOI:

09:31 min

April 27, 2015

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Chapitres

  • 00:05Titre
  • 01:04Gold Nanorods Preparation
  • 02:04NG108-15 Neuronal Cell Line Culture and Differentiation
  • 03:23Neurite Outgrowth Enhancement
  • 05:07Laser-induced Intracellular Ca2+ Imaging
  • 07:50Results: Representative Images of Differentiated NG108-15 Neuronal Cells
  • 09:09Conclusion

Summary

Traduction automatique

This protocol outlines how to use the transient heating associated with the optical absorption of gold nanorods to stimulate differentiation and intracellular calcium activity in neuronal cells. These results potentially open up new applications in neural prostheses and fundamental studies in neuroscience.

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