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पोस्ट-transcriptionally विनियमित जीन का रासायनिक Modulators के लिए उच्च throughput स्क्रीनिंग
Journal JoVE
Biologie
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Journal JoVE Biologie
High-throughput Screening for Chemical Modulators of Post-transcriptionally Regulated Genes
DOI:

09:44 min

March 03, 2015

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Chapitres

  • 00:05Titre
  • 01:30Day 1: Prepare and Seed Cells
  • 02:44Day 2: Treat the Cells with Library Compounds
  • 04:19Day 3: Luciferase Assay
  • 05:27Counter-screening
  • 07:09Results: Modulators of the 3′ UTR of MYCN
  • 09:11Conclusion

Summary

Traduction automatique

Here we describe a cell-based reporter gene assay as a valuable tool to screen chemical libraries for compounds modulating post-transcriptional control mechanisms exerted through 3’ UTR.

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