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Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding
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Journal JoVE Bio-ingénierie
Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

Generation of a Three-dimensional Full Thickness Skin Equivalent and Automated Wounding

DOI:
10.3791/52576-v

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08:35 min

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February 26, 2015

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, , , ,
1Department for Tissue Engineering and Regenerative Medicine,University Hospital Würzburg, 2Translational Center Würzburg, Regenerative Therapies in Oncology and Musculoskelettal Disease,Würzburg Branch of the Fraunhofer-Institute Interfacial Engineering and Biotechnology, IGB

Chapitres

  • 00:05Titre
  • 01:55Production of Dermal Component
  • 03:36Addition of Human Epidermal Keratinocytes and Culture of Full Thickness Skin Equivalents
  • 05:14Injury with Automated Wounding Device
  • 06:19Results: (Immuno-)Histological Analysis and Wounding of Full Thickness Skin Equivalents
  • 08:10Conclusion

Summary

Traduction automatique

Traduction automatique

The goal of this protocol is to build up a three-dimensional full thickness skin equivalent, which resembles natural skin. With a specifically constructed automated wounding device, precise and reproducible wounds can be generated under maintenance of sterility.

Tags

Keywords: 3D Skin EquivalentFull Thickness Skin ModelIn Vitro Wound HealingHuman Dermal FibroblastsHuman Epidermal KeratinocytesCollagen Type I HydrogelAutomated Wounding DeviceStandardized Wounds

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