/
/
Novel Microscopia de Força Atômica bioprospecção Based para Isolamento de Morfologia reagentes específicos contra TDP-43 variantes em Esclerose Lateral Amiotrófica
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Chapitres
- 00:05Titre
- 02:4712 Rounds of Negative Panning Against Bovine Serum Albumin (BSA)
- 04:19AFM Process
- 06:268 Rounds of Negative Panning Against Healthy Immunoprecipitated TDP-43
- 07:54Positive Panning against Immunoprecipitated TDP-43 from Individuals with ALS
- 11:17Results: Analysis of Potential ALS Clones
- 12:59Conclusion
Using atomic force microscopy in combination with biopanning technology we created a negative and positive biopanning system to acquire antibodies against disease-specific protein variants present in any biological material, even at low concentrations. We were successful in obtaining antibodies to TDP-43 protein variants involved in Amyotrophic Lateral Sclerosis.
