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Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
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Biologie
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Journal JoVE Biologie
Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

DOI:
10.3791/52586-v

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10:28 min

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April 14, 2015

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, , , ,
1Diabetes and Islet Biology Group, NHMRC Clinical Trials Centre, Faculty of Medicine,The University of Sydney, 2Biomarkers Laboratory, NHMRC Clinical Trials Centre, Faculty of Medicine,The University of Sydney

Chapitres

  • 00:05Titre
  • 01:29Probe-based RTqPCR Mature MicroRNA (miRNA) Detection and Microfluidics Array Card Preparation
  • 03:40Microfluidics Card Loading and qPCR
  • 04:59Nanofluidics Array Loading and qPCR
  • 08:05Results: Representative miRNA Profile
  • 09:38Conclusion

Summary

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Traduction automatique

Circulating microRNAs have recently emerged as promising and novel biomarkers for various cancers and other diseases. The goal of this article is to discuss three different probe-based real-time PCR platforms and methods that are available to quantify and determine the abundance of circulating microRNAs.

Tags

Probe-based QPCRMicroRNA QuantificationTranscript AbundanceHigh-throughput PlatformsMicrofluidicsNanofluidics ArraysTaqMan Array CardOpenArraySerumPlasma-EDTA

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