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Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer
Journal JoVE
Médecine
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Journal JoVE Médecine
Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer

Utilizing Murine Inducible Telomerase Alleles in the Studies of Tissue Degeneration/Regeneration and Cancer

DOI:
10.3791/52599-v

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08:34 min

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April 13, 2015

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, , , , , ,
1Department of Cancer Biology,UT MD Anderson Cancer Center, 2Novartis Institutes for Biomedical Research, 3Sanofi US, 4Institute of Applied Cancer Science,UT MD Anderson Cancer Center

Chapitres

  • 00:05Titre
  • 01:35Reactivation of Telomerase in mTERT-ER and LSL-mTERT Mice In Vivo
  • 02:33Isolation of Neural Stem Cells and Reactivation of Telomerase In Vitro
  • 05:07Telomere FISH
  • 06:37Results: Effect of Telomerase Reactivation
  • 08:01Conclusion

Summary

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Traduction automatique

Telomere and telomerase play essential roles in ageing and tumorigenesis. The goal of this protocol is to show how to generate two murine inducible telomerase knock-in alleles and how to utilize them in the studies of tissue degeneration/regeneration and cancer.

Tags

Keywords: Telomere DysfunctionTelomerase ReactivationCancerNeural Stem CellsMurine Models4-Hydroxytamoxifen (4-OHT)Lox-Stopper-LoxTERT (LSL-mTERT)Telomere FISHMetaphase Chromosomes

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