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Pairwise crescita Concorso test per la determinazione della Fitness replica del virus dell'immunodeficienza umana
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Chapitres
- 00:05Titre
- 01:23Growth Competition
- 05:00Determination of Viral Ratios
- 09:16Results: HIV Replication Fitness Determined by Pairwise Growth Competition Assays
- 10:54Conclusion
Growth competition between nearly isogenic viruses provides a sensitive measurement for determining relative replication fitness. The protocols described here include the construction of recombinant HIV-1 clones, virus propagation and growth competition and analysis methods optimized to yield sensitive and consistent results.
