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Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence
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Biologie
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Journal JoVE Biologie
Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

Enhanced Genetic Analysis of Single Human Bioparticles Recovered by Simplified Micromanipulation from Forensic ‘Touch DNA’ Evidence

DOI:
10.3791/52612-v

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11:49 min

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March 09, 2015

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, ,
1Forensic Science Graduate Program, Biochemistry Track,University of Central Florida, 2Department of Chemistry,University of Central Florida, 3National Center for Forensic Science,University of Central Florida

Chapitres

  • 00:05Titre
  • 02:13Collection of Bioparticles from Touched Objects and Surfaces
  • 04:31Optional Staining of Bioparticles to Aid in Visualization
  • 05:33Isolation of Bioparticles of Interest for Analysis
  • 09:05Combined Microvolume Nucleic Acid Isolation and Autosomal STR Profiling
  • 09:51Results: Enhanced Genetic Analysis of Touch DNA Evidence
  • 11:14Conclusion

Summary

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Here we describe an optimized and efficient removal strategy for the collection of bio-particles present in ‘touch DNA’ samples, together with an enhanced amplification protocol involving a one-step 5 µl micro-volume lysis/STR amplification, to permit the recovery of short tandem repeat (STR) profiles of the bio-particle donor(s).

Tags

Forensic DNA AnalysisTouch DNAMicromanipulationSingle-source DNA ProfilesSTR AmplificationGenetic AnalysisBioparticle RecoveryEnhanced TechniquesTrace Biological Material

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