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Einfache Polyacrylamid-basierten Multiwell Steifigkeit Assay für das Studium der Steifigkeit abhängige Zell-Antworten
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Chapitres
- 00:05Titre
- 01:33Preparation of Hydrogel-associated Solutions and Aliquots
- 02:41Hydrogel Preparation
- 04:29Multiwell Plate Assembly, Collagen Coating, and Sterilization
- 06:00Results: Polyacrylamide Gel Preparation in Multiwell Plates for Stiffness-dependent Cell Response Studies
- 07:19Conclusion
Here, a method that enables quick, efficient, and inexpensive preparation of polyacrylamide gels in a multiwell plate format is described. The method does not require any specialized equipment and could be easily adopted by any research laboratory. It would be particularly useful in research focused on understanding stiffness-dependent cell responses.
