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3'LIFE의 분석을 사용하여 높은 처리량 miRNA의 표적의 탐지
Journal JoVE
Biologie
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Journal JoVE Biologie
Detection of miRNA Targets in High-throughput Using the 3’LIFE Assay
DOI:

12:49 min

May 25, 2015

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Chapitres

  • 00:05Titre
  • 01:30Preparations Prior to Transfection
  • 02:52Transfection
  • 06:32Cell Lysate Preparation
  • 07:27Dual Luciferase Assay
  • 10:39Results: miRNA Targets are Identified by the 3’LIFE Assays
  • 12:25Conclusion

Summary

Traduction automatique

Luminescent identification of functional elements in 3’ untranslated regions (3’UTRs) (3’LIFE) is a technique to identify functional regulation in 3’UTRs by miRNAs or other regulatory factors. This protocol utilizes high-throughput methodology such as 96-well transfection and luciferase assays to screen hundreds of putative interactions for functional repression.

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