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세균의 사체 분석 사례 연구에 대한 빠르고 안정적​​인 파이프 라인 : 세린에 의존하는 유전자 조절에<em> 폐렴 구균</em
Journal JoVE
Biologie
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Journal JoVE Biologie
A Fast and Reliable Pipeline for Bacterial Transcriptome Analysis Case study: Serine-dependent Gene Regulation in Streptococcus pneumoniae
DOI:

10:18 min

April 25, 2015

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Chapitres

  • 00:05Titre
  • 01:21Isolation and Cleanup of Total RNA
  • 02:58cDNA Preparation and Labeling
  • 04:54cDNA Hybridization
  • 06:59Slide Washing
  • 08:02Results: Bacterial Transcriptome Analysis through Serine-dependent Gene Regulation
  • 09:39Conclusion

Summary

Traduction automatique

This manuscript describes the use of state-of-the-art technology provided by DNA-microarrays. Microarrays provide an overview of the transcriptomic changes in bacteria incurred under a specific condition. Moreover, we highlight the ease by which large amounts of data can be analyzed by using convenient in-house developed software packages.

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