/
/
Захват восстановления тканей в данио рерио Личинки с Интерв светлого стереомикроскопия
This content is Free Access.
Chapitres
- 00:00Titre
- 01:34Raising of Zebrafish to Larval Stages
- 02:26Preparation of the Imaging Chamber
- 03:57Mounting and Imaging of the Pre-injured Larvae
- 05:41Amputation Assay
- 06:00Mounting the Larva for Time-lapse Imaging
- 07:28Time-lapse Imaging
- 09:14Data Analysis
- 11:51Representative Results
- 13:11Conclusion
We present a protocol for capturing the dynamics of zebrafish larval tail fin regeneration on a whole-tissue scale using brightfield-based stereomicroscopy. This technique enables capturing the regeneration dynamics with single cell resolution. This methodology can be adapted to any stereomicroscope equipped with a CCD camera and time-lapse software.
