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Оценка опухоли инфильтрирующие лейкоцитов подмножеств в подкожной опухоли модели
Journal JoVE
Médecine
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Journal JoVE Médecine
Evaluation of Tumor-infiltrating Leukocyte Subsets in a Subcutaneous Tumor Model
DOI:

07:49 min

April 13, 2015

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Chapitres

  • 00:05Titre
  • 01:29Preparing for Sample Collection and Processing
  • 02:15Tumor Resection and Preparation of Single Cell Tumor Suspension
  • 05:47Results: FACS Staining of Single Cell Suspension and Analysis of Leukocyte Subsets
  • 07:21Conclusion

Summary

Traduction automatique

This protocol describes a method for the detailed evaluation of leukocyte subsets within the tumor microenvironment in a mouse tumor model. Chemerin-expressing B16 melanoma cells were implanted subcutaneously into syngeneic mice. Cells from the tumor microenvironment were then stained and analyzed by flow cytometry, allowing for detailed leukocyte subset analyses.

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